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Fig. 4 | BMC Microbiology

Fig. 4

From: Evaluation of PacBio sequencing for full-length bacterial 16S rRNA gene classification

Fig. 4

Alpha diversity analysis using 97% (0.03) OTU distance. Several different alpha diversity indexes: number of observed OTUs a; Chao community richness b; Shannon diversity index c; Q statistic – Qstat diversity index d; inverse Simpson diversity index e; and sample coverage index f calculated in Mothur are shown for five different datasets analyzed. Lines connect the same samples in each detection method. Statistical analysis was performed in Graph Pad using the One-way ANOVA for between group comparisons. Abbreviations: OTU = operational taxonomy unit, MiSeq = Illumina MiSeq sequencing platform, V1V2 = bacterial 16S rRNA gene region V1 to V2, V1V9 = bacterial full-length 16S rRNA gene, LO = PacBio datasets with low stringency post alignment screening using a minimum alignment score of 80% and minimum alignment similarity score of 80%. HI = PacBio datasets with high stringency post alignment screening using a minimum alignment score of 90% and a minimum alignment similarity score of 90%. Dashed line = significant difference at 95% confidence interval (P < 0.05). § = These datasets did not pass the normality test

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