Table 1 The elemental mixtures reproducing metal concentrations and pH of 1h and 24h M. avium phagosome
Supplement | Elemental mix added to 500 ml of Middlebrook 7H9 broth | |
|---|---|---|
1h (pH 6.6) | 24h (nM, pH 5.8) | |
1 M potassium chloride | 14.7 ml | 0.925 ml (1.79) |
1 M calcium chloride | 2 ml | 1.25 ml (2.42) |
1 M manganese chloride | 5.9 ml | 11.9 ml (23) |
1 M copper sulfate | 1.85 μl | 5.5 μl (0.01) |
1 M zinc chloride | 33 μl | 58.7 μl (0.11) |
0.25 M ferric pyrophosphate | 288 μl | 2 ml (0.97) |
1 M nickel chloride | 5 μl | 5 μl (0.01) |