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Table 1 The elemental mixtures reproducing metal concentrations and pH of 1h and 24h M. avium phagosome

From: Identification of Mycobacterium avium subsp. hominissuis secreted proteins using an in vitro system mimicking the phagosomal environment

Supplement Elemental mix added to 500 ml of Middlebrook 7H9 broth
1h (pH 6.6) 24h (nM, pH 5.8)
1 M potassium chloride 14.7 ml 0.925 ml (1.79)
1 M calcium chloride 2 ml 1.25 ml (2.42)
1 M manganese chloride 5.9 ml 11.9 ml (23)
1 M copper sulfate 1.85 μl 5.5 μl (0.01)
1 M zinc chloride 33 μl 58.7 μl (0.11)
0.25 M ferric pyrophosphate 288 μl 2 ml (0.97)
1 M nickel chloride 5 μl 5 μl (0.01)