Inactivation of MSMEG_0412 gene drastically affects surface related properties of Mycobacterium smegmatis

BMC Microbiology

Table 1 Synthetic oligonucleotides

Name	Sequence (5′ - 3′)^a	Position of Annealing^b
0412-S	CATGATCCCCGAGGAGCAC	+857/+875
0412-A	TTGCCGTTCAAGTACCTCGG	+889/+90
0412-Fw	AAAGCTAGCATGAGGAGACGACTTCTCGCGTTC	+1/+24
0412-spFw	AAAGCTAGCGATGACACCAAACCGGTCG	+85/+103
0412-Rv	AAAGCTAGCTCAGGGCTTGGGCGCCGGTTG	+1132/+1152
0412-gfpRv	AAAGCTAGCGGGCTTGGGCGCCGGTTG	+1132/+1149
0412-1F	CTAGAAGCTTGCACCGCACTGGTGAGATA	-690/-672
0412-1R	CTAGGGTACCCGCTGTAGTCCTCTGCT	-5/+12
0412-2F	CTAGGGTACCGGACTGCAGTACGTCGTGAACA	+1108/+1129
0412-2R	CTAGGGTACCACCATGTGGATCCCTCTCCT	+1818/+1837

^aUnderlined is an unpaired tail carrying a restriction site
^bPosition of annealing refers to msmeg_0412 gene sequence, with the first base of the translational initiation codon as +1

ISSN: 1471-2180