Fig. 5
Real-Time PCR analysis of msmeg_0412 gene under different growth conditions. Cells of M. smegmatis mc2155 wild type strain were grown in aerated conditions in minimal medium containing glucose, tributyrin or tween 80 to early exponential growth phase. The ∆∆Ct method was used to calculate the relative amount of specific RNA present in each sample, and the transcriptional induction determined by comparison to values from cells growing in minimal medium containing glucose