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Table 1 Bacterial strains and plasmids used in this study

From: OxyR-regulated catalase CatB promotes the virulence in rice via detoxifying hydrogen peroxide in Xanthomonas oryzae pv. oryzae

Strain or plasmid Relevant characteristicsa Source or Reference
Escherichia coli
 DH5α supE44 ΔlacU169(Φ80lacZΔM15) hsdR17 recA1 endA1 gyrA96 thi-1 relA1 Hanahan 1983 [47]
 BL21 For protein expression Novagen
Xanthomonas oryzae pv. oryzae
 PXO99A Wildtype strain, Philippine race 6 Lab collection
 ∆catB catB gene deletion mutant derived from PXO99A, Gmr This study
 ∆catB(pBBR-catB) Complementary bacterium strain of ∆catB, Apr This study
 ∆oxyR oxyR gene deletion mutant derived from PXO99A, Gmr Our lab
 ∆oxyR(pBBR-oxyR) Complementary bacterium strain of ∆oxyR, Apr Our lab
Plasmid
 pMD18-T Cloning vector, Apr TaKaRa, Tokyo
 pET-28a Expression vector to generate a N-terminal His6 tag, Kmr Haigene
 pK18mobsacB Suicidal vector carrying sacB gene for mutagenesis, Gmr Schafer et al., 1994 [44]
 pBBR1MCS-4 Broad-host range expression vector, Apr Kovach et al., 1995 [48]
 pHM1 Broad-host range expression vector, Spr Hopkins et al., 1992 [49]
 pHT304BZ Promoterless lacZ vector, Apr Lereclus et al., 1996 [50]
 pMDcatB pMD18-T derivative carrying the full length of catB, Apr This study
 pMDcatBr pMD18-T derivative carrying the right fragment of catB, Apr This study
 pMDcatBl pMD18-T derivative carrying the left fragment of catB, Apr This study
 pMDoxyR pMD18-T derivative carrying the full length of oxyR, Apr This study
 pKcatB pK18mobsacB derivative carrying the full length of catB, Gmr This study
 pEToxyR pET-28a derivative carrying the full length of oxyR, Kmr This study
 pBBR-catB pBBR1MCS-4 derivative carrying the full length of catB, Apr This study
 pHTpB pHT304BZ derivative carrying the promoter region of catB, Apr This study
 pH-lacZ pHM1 derivative carrying the promoterless lacZ, Spr This study
 pH-catBp-lacZ pHM1 derivative carrying the promoter region of catB and promoterless lacZ, Spr This study
  1. aApr,Kmr,Spr,and Gm rindicate resistant to ampicillin, kanamycin, spectinomycin and gentamicin, respectively