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Fig. 5 | BMC Microbiology

Fig. 5

From: OxyR-regulated catalase CatB promotes the virulence in rice via detoxifying hydrogen peroxide in Xanthomonas oryzae pv. oryzae

Fig. 5

Assays for catalase activity and H2O2 accumulation of Xanthomonas oryzae pv. oryzae strains. a Catalase activity assays. The cells of wildtype, ∆catB, ∆oxyR, ∆catB(pBBR-catB) and ∆oxyR(pBBR-oxyR) were disrupted by sonication, and the cell extracts were separated by centrifugation at 12,000 g. Catalase activities associated with cell extracts were assessed by spectrophotometric assay. One unit (U) is defined as the amount of activity required to decompose 1 μmol of H2O2 in one minute. b H2O2 accumulation assays. The H2O2 concentrations of Xoo strains were measured as described in Methods, and the account of H2O2 was accounted by the standard curve. Error bars represent standard derivations from three replicates, and different letters above the bars denote statistically significant differences (P < 0.05, Student’s t test)

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