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Fig. 4 | BMC Microbiology

Fig. 4

From: OxyR-regulated catalase CatB promotes the virulence in rice via detoxifying hydrogen peroxide in Xanthomonas oryzae pv. oryzae

Fig. 4

Assays for H2O2 resistance of Xanthomonas oryzae pv. oryzae strains. a Disk diffusion assays. Wildtype, ∆catB, ∆oxyR, ∆catB(pBBR-catB) and ∆oxyR(pBBR-oxyR) strains at OD600 of 1.0 were mixed with PSA medium at 1 : 100 v/v and disks saturated with different concentrations (0.25, 0.5 and 1 M) of H2O2 were placed on the central of plates. These plates were incubated at 28 °C for 72 h and the H2O2 inhibition zones were observed. b Diameters of the H2O2 inhibition zones. c H2O2 sensitivity assays. Wildtype, ∆catB,oxyR,catB(pBBR-catB) and ∆oxyR(pBBR-oxyR) strains at OD600 of 1.0 were mixed with fresh M210 liquid medium at 1: 1000 v/v, and the H2O2 were added to the final concentration at 0, 0.25, 0.5 and 1 mM, respectively. The mixtures were incubated at 28 °C with 200 rpm, and the bacterial population were detected at 12 and 24 h. These experiments repeat three times, independently. Error bars represent standard derivations, and different letters above the bars denote statistically significant differences (P < 0.05, Student’s t test)

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