The highly variable microbiota associated to intestinal mucosa correlates with growth and hypoxia resistance of sea bass, Dicentrarchus labrax, submitted to different nutritional histories

Table 1 Pyrosequencing yield and mean indices of bacterial diversity in the experimental groups of sea bass

	LH1-LH2	C1-LH2	C1-C2	C1-HG2	HG1-HG2	p-value
Valid reads per sample before normalization	17634^ab ± 1052	20894^a ± 1006	16603^b ± 867	18492^ab ± 1135	20603^a ± 900	0.01
Diversity index after normalization:
OTU richness	45.6 ± 3.7	50.7 ± 5.1	45.4 ± 3.6	47.3 ± 3.4	49.3 ± 2.9	0.83
Dominance	0.31 ± 0.03	0.38 ± 0.05	0.30 ± 0.03	0.41 ± 0.05	0.33 ± 0.03	0.21
Intra-group similarity (Bray-Curtis)	0.53^ab ± 0.02	0.42^c ± 0.03	0.52^ab ± 0.02	0.60^a ± 0.02	0.52^b ± 0.02	≤0.001

The means (± SE) were compared by ANOVA; in case of significant difference, superscript letters on the same line indicated the significant differences after post-hoc Tukey’s test. The diversity indices were computed after normalizing the numbers of total reads per sample, based on the minimum observed (11,599 reads). Each group was named after its diets during the two challenge phases: HUFA-deficient diet at both phases (LH1-LH2), or only at phase 2 (C1-LH2); hypoproteic diet with high starch supply at both phases (HG1-HG2), or only at phase 2 (C1-HG2); control with standard diets (C1-C2)

ISSN: 1471-2180