Fig. 6
From: CP40 from Corynebacterium pseudotuberculosis is an endo-β-N-acetylglucosaminidase
CP40 hydrolyzes biantennary, but not bisecting glycans on IgG glycans from human and ovine IgGs. Glycans were released from human and ovine IgG by PNGase F, EndoS and CP40 respectively, glycans were with 2AB and analyzed by HILIC. The structures annotated according to the digested glycan using a series of defined exoglycosidase. The released biantennary glycans from IgG and the cleavage site of the glycan chain are shown. Monosaccharide identities are shown to the right in the middle and conform to the standard colors and shapes as devised by the Consortium for Functional Glycomic (www.functionalglycomics.org)