Fig. 1

CP40 is similar to bacterial endoglycosidases. a. The amino acid sequence of CP40 was aligned with a number of endoglycosidases and serine proteases from different bacterial species. CP40 is enclosed by a rectangle. A phylogenetic tree was reconstructed using the neighbor-joining method, validated by bootstrapping (10,000 replications) generating a consensus tree. Numbers on branching points shows the percentage of resampling trees supporting the consensus tree. GenBank accession numbers of the proteins are indicated in front of the name of each enzyme. The asterisks indicate the serine proteases. b. The putative family 18 glycosyl hydrolase active site of CP40 was aligned with the verified active sites of EndoE from E. faecalis and EndoS from S. pyogenes