Fig. 6

Effects of SpxB upon detergent-induced lysis. a WU2 and T4R along with their ∆spxB, ∆lytA, and ∆spxB/∆lytA mutants were grown to an OD of 0.5. Equal volumes of sterile supernatants were run on SDS-PAGE gels and probed for PLY via western blot. Each figure represents three independent experiments ± SD. (*p < 0.05). A representative western blot image for each strain is shown. b T4R and its ∆spxB mutant were grown to an OD of 0.5 prior to treatment with 0.05 % sodium deoxycholate (DOC). Spectrophotometric readings were taken every minute for 10 min to determine differences in the rate of induced autolysis