Fig. 4
From: Pyruvate oxidase of Streptococcus pneumoniae contributes to pneumolysin release
SpxB-dependent PLY release is not due to decreased transcription of the ply gene. Strains AW267 (a), T4R (b), WU2 (c), and their respective ∆spxB mutants were grown to mid-log phase and RNA was isolated and used for cDNA synthesis followed by qRT-PCR. GyrA was utilized as an internal control housekeeping gene. Fold-changes were quantitated by the 2∆∆Ct method. Each figure represents three independent experiments ± SD