Fig. 3
From: Pyruvate oxidase of Streptococcus pneumoniae contributes to pneumolysin release
H2O2 production correlates with PLY release in clinical isolates. The amount of H2O2 and PLY in culture supernatants from 15 clinical isolates was measured by a colorimetric assay and dot blot, respectively. Dot intensities were quantiated using densitometry. PLY concentrations were interpolated using a standard line generated by serial dilution of recombinant PLY. Values for H2O2 and PLY were normalized to cell counts to account for differences in cell counts between strains and plotted versus each other. A linear regression was generated yielding a significant correlation (p < 0.05, r 2 = 0.3167)