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Fig. 2 | BMC Microbiology

Fig. 2

From: Pyruvate oxidase of Streptococcus pneumoniae contributes to pneumolysin release

Fig. 2

Deletion of spxB greatly reduces PLY observed in the supernatant. a-d The amount of PLY released into culture supernatants from parental and mutant strains lacking SpxB (a AW267; b WU2, c T4, and d T4R) was measured by western blot. Band intensities were quantitated by densitometry. Results are representative of three independent experiments ± SD. (* p < 0.05, ** p < 0.005, **** P < 0.00005). Where indicated, the cultures were complemented with 10 ng of catalase. e and f The amount of PLY released in culture supernatants from parental, mutant strains lacking SpxB, and complemented strains (e T4; f T4R) was measured by western blot. Band intensities were quantitated by densitometry. Results are representative of three independent experiments ± SD. (* p < 0.05, ** p < 0.005, *** P < 0.0005). Representative images of western blots are shown

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