Fig. 2
From: Spontaneous mutations in the flhD operon generate motility heterogeneity in Escherichia coli biofilm
PCR1 and PCR2. a details the flhD operon and the two PCR reactions [11]. PCR1 is expected to yield a 1199 bp product, PCR2 is expected to yield a 1344 bp product in the absence of insertions or deletions. FP1stands for Forward Primer for PCR1, FP2 stands for Forward Primer for PCR2, RP stands for Reverse Primer for both PCR1 and PCR2. IS5 refers to the IS5 element at position −99 to −96 from the transcriptional start that was described by Barker et al. [11] and Wang and Wood [12]. IS1 refers to the IS1 elements that was described by Lee and Park [13] at −303 pb. IS refers to the IS5 described by Fahrner and Berg as the most distal (−476) IS element that had a positive effect on flhD expression [14]. b contains the 1 kb ladder (Amresco, Solon OH) and 14 lanes of PCR products: lanes 1 & 2, MC1000; lanes 3 & 4, JS81; lanes 5 & 6, JS45; lanes 7 & 8, JS56; lanes 9 & 10, JS87; lanes 11 & 12, JS44; lanes 13 and 14, JS43. Odd numbered lanes show products of PCR1, even numbers lanes those of PCR2