Fig. 1From: Spontaneous mutations in the flhD operon generate motility heterogeneity in Escherichia coli biofilmCompetitive biofilm experiment. MC1000 and BP19 were grown at a starting ratio of 1:1. Biofilm bound bacteria were enumerated on LB and LB/kn plates. The total number of bacteria (white diamonds, dashed line) is presented as colony forming units per well (CFU/well) and was calculated from the LB plates. To determine the percentage of flhD mutants (black squares, solid line), bacterial counts from the kanamycin plates were divided by total bacterial numbers. Dashed lines mark 50 and 100 % flhD mutantsBack to article page