From: Bacterial genome engineering and synthetic biology: combating pathogens
Engineering tool | Methodology | Engineered pathogenic strains |
---|---|---|
Recombineering | Homologous recombination of linear DNA utilizing λ-Red enzymes Gam, Exo and Bet |
Salmonella enterica [26] a
Escherichia coli [23] a Pseudomonas aeruginosa [29] a Streptomyces coelicolor [28] b Shigella dysenteriae [50] a |
pORTMAGE |
Portable Multiplex Automated Genome Engineering (MAGE) Utilizing recombineering | Salmonella enterica [35]a |
Targetrons | Retrohoming of Mobile Group II Introns by reverse splicing and insertion in genome |
Clostridium perfringens [38] b
Vibrio Cholera [78] a Yersinia pseudotuberculosis [79] a Staphylococcus aureus [80]b |
Phage Engineering CRISPR/Cas9 | Delivery of CRISPR genes and RNA guides for sequence specific antimicrobials |
Carbapenem-resistant Enterobacteriaceae Enterohemorrhagic Escherichia coli [55] a |
Antisense RNA | Post-transcriptional gene silencing |
Staphylococcus aureus [81] b
Streptomyces coelicolor [82] b |