Emerging Helicobacter pylori levofloxacin resistance and novel genetic mutation in Nepal

BMC Microbiology

Table 4 MIC of metronidazole resistant strains and the mutation of rdxA and frxA genes

No	Strains	MIC (mg/L)	rdxA	frxA
1	2	48	13frameshift	R86^a
2	4	>256	Q11^a	18frameshift
3	5	64	N73^a	R3T, 54frameshift
4	8	64	Q11^a	Q5^a
5	14	32	R16H, L62V, K190^a	P2E, R3P, M66I, A70V
6	15	64	K2N, 4frameshift	I44T, 47frameshift
7	16	48	E107R, 109frameshift	W137^a
8	18	128	C148Y	undetermined
9	29	>256	R16H, A80T, S108A	18frameshift
10	34	64	undetermined	106frameshift
11	41	16	R16H, R41K, 43frameshift	G76R, A152V
12	49	>256	C140Y	18frameshift
13	52	>256	G189S	undetermined
14	55	24	L62V, S108A, S196N, Q197^a	P41L, E176K
15	61	12	None	A15V, I144V, M66I
16	64	32	R16H, S108A, R176C, S196N	None
17	70	32	K60^a	D2E, A85V, K178N
18	74	64	S45G	6frameshift
19	83	>256	M21V, A80T, Q119^a	A70V
20	84	16	Q50^a	R58H
21	86	96	Q50^a	R25T, M66I, A154T
22	89	32	Q65^a	A115V
23	90	64	45frameshift	18frameshift
24	92	>256	C140Y	18frameshift
25	94	64	Q50^a	undetermined
26	108	128	R16L	18frameshift
27	110	32	None	P41L
28	113	128	A40T	A16T, I44V, 70frameshift
29	114	>256	Q16^a	18frameshift
30	116	>256	G163D	18frameshift
31	120^b	>256	None	None
32	123	>256	Q50^a	V6^a
33	124	>256	D23G	18frameshift
34	137	128	M56I, 201frameshift	70frameshift
35	140	96	60frameshift	71frameshift
36	141	64	S43L	72frameshift
37	142	64	undetermined	A15V

Q11^a means premature stop codon at Gln11; 13frameshift means frameshift mutation in the amino acid 13; R16H means amino acid substituted at Arg-16; None means no specific mutation; Undetermined is the strains that failed to show identifiable specific bands of rdxA or frxA target in PCR
^bHigh MIC values strain without specific mutation in rdxA and frxA but contained mutation in dppA and dapF

ISSN: 1471-2180