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Fig. 1 | BMC Microbiology

Fig. 1

From: N-acetyl-cysteine exhibits potent anti-mycobacterial activity in addition to its known anti-oxidative functions

Fig. 1

NAC reverts M. tuberculosis-induced oxidative stress. Cryopreserved heparinized plasma samples collected from active pulmonary TB (PTB; n = 30), latent TB individuals (LTBI; n = 20) and healthy controls (HC; n = 20) from Salvador Brazil were used in these studies. Total oxidant status (a), lipid peroxidation (b) and total antioxidant status (c) were measured as described in Methods. d-f Primary human monocyte-derived macrophages were infected with H37Rv M. tuberculosis at MOI of 5 and treated or not with NAC (10 mM). Lipid peroxidation (d), DNA oxidation (e) and cell death (f) were assessed at indicated time points post-infection (p.i.) as described in Methods. g Intracellular production of ROS in primary human macrophages infected with H37Rv M. tuberculosis at different MOI was measured by flow cytometry. ROS production was also verified in infected-macrophages after NAC treatment (10 mM). Results were plotted as histograms where the mean fluorescence intensity (MFI) was compared between the experimental groups. In a-c lines represent median values. In d-g data represent means ± SEM of triplicate samples from a minimum of six donors. In a-f data were analyzed using Kruskal-Wallis test with Dunn’s multiple comparisons post-test. In g MOI titration data were compared using Kruskal-Wallis test with non-parametric linear trend post-test. The effect of NAC treatment was analyzed using the Mann-Whitney U test. The data shown are representative of three independent experiments. (*p <0.05; **p <0.01, ***p < 0.001)

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