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Table 4 Detection of atrazine degradation genes in the isolates and ribosomal diversity of the strains representing separate ERIC types

From: Occurrence, diversity and community structure of culturable atrazine degraders in industrial and agricultural soils exposed to the herbicide in Shandong Province, P.R. China

ERIC type Genes for atrazine degradation Strains (GenBank Accession No.)a Nearest type strain (GenBank Accession No.) [Percent similarity]b
A trzN atzBC D5-2 (KF889364), D5-4, D5-9, D5-10, D5-14, D5r1-1 (KF889366), D5r1-3, D5r1-4, D5r2-1, D5r2-3, D5rh3-1, D5r3-3, D5r3-4, D5r3-5, D6-2 (KF889365), D6-4, D6-5, D6r1-1 (KF889367), D6r1-3, D6r1-4, D6r2-1, D6r2-2, D6r2-3, D6r2-5 (KF889368), D6r3-1, D6r3-2, D6r3-3, D6r3-4 Arthrobacter nitroguajacolicus G2-1T (NR_027199) [99.3–99.4 %]
trzN atzC D6r1-2
B trzN atzBC D5r1-2 (KF889369), TD1-1, TD1-2, TD1-3, TD1-4, TD2-2, TD2-4 (KF889370), TD3-1, TD3-2, TD3-3, TD4-1 (KF889371), TD 4–2, TD5-1, TD6-1, TD6-3; DnW1-1 (KF889372), DnW1-2, DnW2-1, DnW2-2, DnW3-1, DnW3-2; DnL1-1 (KF889373), DnL1-3, DnL2-1, DnL3-1, DnL3-3; GD1-1 (KF889374), GD1-4, GD2-2, GD2-3, GD3-1, GD3-2, GD3-3, GD3-4, GD3-5; WS1-1, WS2-3 Arthrobacter ureafaciens NCT (NR_029281) [99.5– 99.6 %]
trzN D5r2-2, TD2-1, TD6-2, DnL1-2, DnL2-2, DnL3-2, WS1-2, WS2-1, WS2-2 (KF889375), WS2-5, WS3-1, WS3-2.
C trzN atzBC TD1-5-1, TD1-5-2 (KF889379), TD2-3 (KF889376), D4-3 (KF889377), TD4-4 (KF889378), TD5-2, TD5-3 Nocardioides panacihumi Gsoil 616T (NR_041518) [98.1–98.3 %]
D trzN atzBC GD1-2, GD1-3 (KF889380), GD2-1, GD2-4, WS1-3 (KF889381), WS2-4 Nocardioides ganghwensis JC2055 T (NR_025776) [99.8 %]
E trzN atzBC D6rh3-5 (KF889382) Arthrobacter sulfonivorans ALLT (NR_025084) [99.4–99.6 %]
trzN atzC D5rh3-2 (KF889383)
F trzN atzBC D3-7, D3-8 (KF889384) Gulosibacter molinativorax ON4T (NR_025451) [100 %]
G atzABC D3-1l (KF889385), D3-1s, D3-4, D3-5 Pseudomonas alcaliphila AL15-21T (NR_024734) [99.1–99.3 %]
atzAC D3-2 (KF889386)
H trzN atzBC D3-3 (KF889387), D3-6 (KF889388) Arthrobacter crystallopoietes DSM 20117T (NR_026189) [99.8 %]
I trzN atzBC D5-3 (KF889389) Arthrobacter crystallopoietes DSM 20117T (NR_026189) [99.6 %]
J trzN atzBC D5-5 (KF889390) Arthrobacter crystallopoietes DSM 20117T (NR_026189) [99.8 %]
K trzN atzBC D5-7 (KF889391) Arthrobacter crystallopoietes DSM 20117T (NR_026189) [99.7 %]
L trzN atzBC D5-12, D5-13 (KF889392) Arthrobacter oxydans DSM 20119T (NR_026236) [99.7–99.8 %]
trzN atzBC D5-8 (KF889393)
trzN D6-1 (KF889394), D6-3, D6-3 s
M trzN atzBC D5-6 (KF889395) Arthrobacter phenanthrenivorans Sphe3T (NR_074770) [99.9 %]
N trzN atzBC D5-11 (KF889396) Arthrobacter subterraneus CH7T (NR_043546) [99.8 %]
O trzN atzBC D6r1-5 (KF889397) Arthrobacter nitroguajacolicus G2-1T (NR_027199) [100 %]
P trzN atzBC D6r2-4 (KF889398) Arthrobacter nitroguajacolicus G2-1T (NR_027199) [99.8 %]
Q trzN atzBC D5-1 (KJ010189) Arthrobacter nitroguajacolicus G2-1T (NR_027199) [99.6 %]
  1. aNames of the strains selected for sequencing of 16S rRNA genes and GenBank accession numbers are typed in bold. Letters in the name of each strain and numbers after letter D represent sampling sites listed in the Table 1. Letter “r” following D5 and D6 means that the strain has been isolated from the rhizosphere. The first digit in the names of strains originated from TD, DnW, DnL, GD and WS sites indicates replicate samples. Site TD(a) was represented by replicate samples 1–3, and site TD(b) – by replicate samples 4–6
  2. b According to BLAST, based on over 1235 bp nucleotide sequences of 16S rRNA genes