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Fig 4 | BMC Microbiology

Fig 4

From: Occurrence, diversity and community structure of culturable atrazine degraders in industrial and agricultural soils exposed to the herbicide in Shandong Province, P.R. China

Fig 4

Detection of atrazine-degrading bacteria in soils by PCRs targeting the genes trzN, atzA, atzB and atzC. Lanes are designated by names of the sampling sites listed in Table 1. Numbers of replicate samples are given below the designations of sampling sites. Site TD(a) was represented by replicate samples 1–3, and site TD(b) by replicate samples 4–6. The primers for trzN, atzA, atzB and atzC were trzN1114f/trzN1271r, atzA655f/atzA982r, atzB181f/atzB316r and atzC340f/atzC552r, respectively. Lanes P contain products of positive control reactions for which template DNAs were isolated from S1 soil supplemented with 104 CFU g−1 of Pseudomonas sp.D3-1l for atzA gene and with 106 CFU g−1 of A. ureafaciens DnL1-1 for trzN and atzC. Positive controls for the gene atzB were reactions with template DNAs isolated from S1 soil aliquots supplemented with known titers of Pseudomonas sp.D3-1l (Additional file 11: Figure S9). Lanes M contain DL 2000 DNA Marker (Takara Biotechnology (Dalian) Co., Ltd., China)

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BMC Microbiology

ISSN: 1471-2180

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