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Table 4 Fucosyllactose utilization, metabolite formation and L-fucose accumulation

From: Fucosyllactose and L-fucose utilization of infant Bifidobacterium longum and Bifidobacterium kashiwanohense

Strain

Substrate

Metabolite formed

 

Supplied

used (mM)

L-fucose (mM)

lactate (mM)

acetate (mM)

1,2-PD (mM)

B. longum subsp. infantis DSM 20088

2′-FL

−24.0 ± 4.4

0.7 ± 1.1

18.0 ± 2.7

29.0 ± 6.6

6.8 ± 1.5

3′-FL

−23.6 ± 0.2

0

14.0 ± 0.8

23.5 ± 0.7

7.1 ± 0.2

B. longum subsp. suis BSM11-5

2′-FL

−20.1 ± 4.1

5.2 ± 0.7

13.1 ± 4.4

13.3 ± 4.8

3.2 ± 0.4

3′-FL

−24.7 ± 3.9

0.5 ± 0.5

10.3 ± 1.3

27.3 ± 7.0

7.5 ± 1.2

B. kashiwanohense DSM 21854

2′-FL

−25.0 ± 0.2

10.5 ± 0.2

12.3 ± 1.0

18.2 ± 1.3

0

3′-FL

−19.9 ± 2.1

10.2 ± 0.1

11.3 ± 0.8

19.2 ± 1.1

0

  1. Strains were grown with 3′-FL or 2′-FL (28 and 27 mM. respectively) as sole carbohydrate source for 48 h. 2′-FL and 3′-FL concentrations were determined with HPAEC-PAD, L-fucose, lactate, acetate, and 1,2-PD were analysed with HPLC-RI (n = 3)
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BMC Microbiology

ISSN: 1471-2180

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