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Table 4 Fucosyllactose utilization, metabolite formation and L-fucose accumulation

From: Fucosyllactose and L-fucose utilization of infant Bifidobacterium longum and Bifidobacterium kashiwanohense

Strain Substrate Metabolite formed
  Supplied used (mM) L-fucose (mM) lactate (mM) acetate (mM) 1,2-PD (mM)
B. longum subsp. infantis DSM 20088 2′-FL −24.0 ± 4.4 0.7 ± 1.1 18.0 ± 2.7 29.0 ± 6.6 6.8 ± 1.5
3′-FL −23.6 ± 0.2 0 14.0 ± 0.8 23.5 ± 0.7 7.1 ± 0.2
B. longum subsp. suis BSM11-5 2′-FL −20.1 ± 4.1 5.2 ± 0.7 13.1 ± 4.4 13.3 ± 4.8 3.2 ± 0.4
3′-FL −24.7 ± 3.9 0.5 ± 0.5 10.3 ± 1.3 27.3 ± 7.0 7.5 ± 1.2
B. kashiwanohense DSM 21854 2′-FL −25.0 ± 0.2 10.5 ± 0.2 12.3 ± 1.0 18.2 ± 1.3 0
3′-FL −19.9 ± 2.1 10.2 ± 0.1 11.3 ± 0.8 19.2 ± 1.1 0
  1. Strains were grown with 3′-FL or 2′-FL (28 and 27 mM. respectively) as sole carbohydrate source for 48 h. 2′-FL and 3′-FL concentrations were determined with HPAEC-PAD, L-fucose, lactate, acetate, and 1,2-PD were analysed with HPLC-RI (n = 3)