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Table 4 Oligonucleotide primers

From: Bacillus subtilis 5′-nucleotidases with various functions and substrate specificities

Name

Sequence (5′–3')a

Restriction site

araL-F

gggaattccatatgcgtattatggccagtcatgat

NdeI

araL-R

ccgctcgagtatcagaatcccctcctcagc

XhoI

yutF-F

cgcggatccatgaaaacatataaagggtattta

BamHI

yutF-R

cccaagcttaatgtatggaatccattcagt

HindIII

ysaA-F

cgcggatccatgaaagccgtattttttgattta

BamHI

ysaA-R

ccgctcgagtttctctaatataggaaacaa

XhoI

YqeG-F

gggaatccatatgttaaaaaagtttttt

NdeI

YqeG-R

cccaagcttttactcctcccactgaat

HindIII

ycsE-F

gggaattccatatgtctgtccaaagagaagatgta

NdeI

ycsE-R

ccgctcgagtagtacccaatggcgaatcgcttta

XhoI

yktC-F

cgcggatccatgacaaattggacggaaatcgat

BamHI

yktC-R

cccaagcttcttccgagcatgaagatactc

HindIII

ycsE-1

gtttattgatggcgtgac

 

ycsE-2

ctgtgtagccttgagagtgatgaacagacatatatgtacctctc

 

ycsE-3

tcactctcaaggctacacag

 

ycsE-4

cgcaagcttcaaaaattatatggag

 

ycsE-5

ctccatataatttttgaagcttgcggggtactataaaaaaagagagtcc

 

ycsE-6

aacacttcatttgcggtc

 

yktC-1

gttcgcctagagctgtaagcttc

 

yktC −2

gccgatgataagctgtcaaagcaatctcatcgatttccgtcc

 

yktC −3

tttgacagcttatcatcggc

 

yktC −4

ttataaaagccagtcattaggc

 

yktC −5

gcctaatgactggcttttataagtgctagccggaaacccatc

 

yktC −6

gaacatctatcaccagacgacatc

 

yqeG-d1

gacataatgcgccatttgatggttg

 

yqeG-d2

gcttgagtcaattccgctgtcgctcaaacgaaaagggcacattcag

 

yqeG-d3

cgacagcggaattgactcaagc

 

yqeG-d4

cgcaagcttacgataaacccagc

 

yqeG-d5

gctgggtttatcgtaagcttgcgcaaaacccgcacccttccc

 

yqeG-d6

caggcagagcatatggatacg

 

yqeG-s1

ccttccagggtatgtttctc

 

yqeG-s2

gatactgcactatcaacacactctttcgacatggatgagcgatgatg

 

yqeG-s3

aagagtgtgttgatagtgcagtatc

 

yqeG-s4

caacaagctggggatccg

 

yqeG-s5

cggatccccagcttgttgcgacaatcattttgaaagaaagaaaaaggg

 

yqeG-s6

gatgacctcgtttccaccggcaaccttttccatttcttactcctcc

 

yqeG-s7

ccggtggaaacgaggtcatc

 

yqeG-s8

cacaaattaaaaactggtctgatcggcctaactcacattaattgcgttg

 

yqeG-s9

cgatcagaccagtttttaatttgtg

 

yqeG-s10

ttaacaaaattctccagtcttcacatcg

 
  1. aRestriction enzyme cleavage sites are underlined