Table 3 Bacterial strains and plasmids
From: Bacillus subtilis 5′-nucleotidases with various functions and substrate specificities
Strain and plasmid | Description | Source or reference |
|---|---|---|
B. subtilis | ||
168 | trpC2 | Laboratory stock |
NON01 | trpC2 ycsE::spc | This study |
NON02 | trpC2 yktC::cat | This study |
NON03 | trpC2 ycsE::spc yktC::cat | This study |
NON04 | trpC2 amyE::(Pspac-yqeG erm) | This study |
NON05 | trpC2 amyE::(Pspac-yqeG erm) ΔyqeG::kan | This study |
NON06 | trpC2 ycsE::spc yktC::cat amyE::(Pspac-yqeG lacI erm) ΔyqeG::kan | This study |
TMO310 | trpC2 aprE::(spec lacI Pspac-mazF) | [62] |
TMO311 | trpC2 aprE::(kan lacI Pspac-mazF) | [62] |
E. coli | ||
DH5α | F− Φ80lacZΔM15 Δ(lacZYA-argF) U169 recA1 endA1 hsdR17(rK −, mK +) phoA supE44 λ− thi-1 gyrA96 relA1 | Laboratory stock |
BL21(DE3) | F− ompT gal dcm lon hsdS B (r B −, m B −) λ(DE3 [lacI lacUV5-T7p07 ind1 sam7 nin5]) [malB +]K-12(λS) | Laboratory stock |
Plasmids | ||
pCRE-test | cat | [63] |
pET28b | kan | Takara Bio |
pET28b-araL | kan, araL | This work |
pET28b-yutF | kan, yutF | This work |
pET28b-yqeG | kan, yqeG | This work |
pET28b-ysaA | kan, ysaA | This work |
pET28b-ycsE | kan, ycsE | This work |
pET28b-yktC | kan, yktC | This work |
pMD20-araL | amp | This work |
pMD20-yutF | amp | This work |
pMD20-yqeG | amp | This work |
pMD20-ysaA | amp | This work |
pMD20-ycsE | amp | This work |
pMD20-yktC | amp | This work |
pMutin2 | erm lacI | [64] |