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Table 3 Bacterial strains and plasmids

From: Bacillus subtilis 5′-nucleotidases with various functions and substrate specificities

Strain and plasmid Description Source or reference
B. subtilis
 168 trpC2 Laboratory stock
 NON01 trpC2 ycsE::spc This study
 NON02 trpC2 yktC::cat This study
 NON03 trpC2 ycsE::spc yktC::cat This study
 NON04 trpC2 amyE::(Pspac-yqeG erm) This study
 NON05 trpC2 amyE::(Pspac-yqeG erm) ΔyqeG::kan This study
 NON06 trpC2 ycsE::spc yktC::cat amyE::(Pspac-yqeG lacI erm) ΔyqeG::kan This study
 TMO310 trpC2 aprE::(spec lacI Pspac-mazF) [62]
 TMO311 trpC2 aprE::(kan lacI Pspac-mazF) [62]
E. coli
 DH5α F Φ80lacZΔM15 Δ(lacZYA-argF) U169 recA1 endA1 hsdR17(rK , mK +) phoA supE44 λ thi-1 gyrA96 relA1 Laboratory stock
 BL21(DE3) F ompT gal dcm lon hsdS B (r B , m B ) λ(DE3 [lacI lacUV5-T7p07 ind1 sam7 nin5]) [malB +]K-12S) Laboratory stock
Plasmids
 pCRE-test cat [63]
 pET28b kan Takara Bio
 pET28b-araL kan, araL This work
 pET28b-yutF kan, yutF This work
 pET28b-yqeG kan, yqeG This work
 pET28b-ysaA kan, ysaA This work
 pET28b-ycsE kan, ycsE This work
 pET28b-yktC kan, yktC This work
 pMD20-araL amp This work
 pMD20-yutF amp This work
 pMD20-yqeG amp This work
 pMD20-ysaA amp This work
 pMD20-ycsE amp This work
 pMD20-yktC amp This work
 pMutin2 erm lacI [64]