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Table 2 Kinetic constants of YktC

From: Bacillus subtilis 5′-nucleotidases with various functions and substrate specificities

Substrate

K M (mM)a

V max (μmol min−1 mg protein−1)a

k cat (s−1)a

MIMP

0.076 ± 0.006

0.82 ± 0.16

2.9 ± 0.33 × 103

IMP

1.1 ± 0.083

0.078 ± 0.0084

1.8 ± 0.59 × 102

AMP

1.8 ± 0.13

0.030 ± 0.00022

5.3 ± 0.039 × 101

GMP

1.9 ± 0.026

0.169 ± 0.028

3.0 ± 0.34 × 102

CMP

1.6 ± 0.017

0.049 ± 0.014

1.5 ± 0.52 × 102

UMP

2.4 ± 0.052

0.11 ± 0.0099

9.8 ± 4.6 × 102

G6P

2.6 ± 0.51

0.021 ± 0.010

3.7 ± 1.3 × 101

β-glycerophosphate

0.49 ± 0.035

1.2 ± 0.32

5.7 ± 1.2 × 102

  1. aAll presented data are the mean values of three independent experiments ± SD