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Table 2 Kinetic constants of YktC

From: Bacillus subtilis 5′-nucleotidases with various functions and substrate specificities

Substrate K M (mM)a V max (μmol min−1 mg protein−1)a k cat (s−1)a
MIMP 0.076 ± 0.006 0.82 ± 0.16 2.9 ± 0.33 × 103
IMP 1.1 ± 0.083 0.078 ± 0.0084 1.8 ± 0.59 × 102
AMP 1.8 ± 0.13 0.030 ± 0.00022 5.3 ± 0.039 × 101
GMP 1.9 ± 0.026 0.169 ± 0.028 3.0 ± 0.34 × 102
CMP 1.6 ± 0.017 0.049 ± 0.014 1.5 ± 0.52 × 102
UMP 2.4 ± 0.052 0.11 ± 0.0099 9.8 ± 4.6 × 102
G6P 2.6 ± 0.51 0.021 ± 0.010 3.7 ± 1.3 × 101
β-glycerophosphate 0.49 ± 0.035 1.2 ± 0.32 5.7 ± 1.2 × 102
  1. aAll presented data are the mean values of three independent experiments ± SD