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Fig. 4 | BMC Microbiology

Fig. 4

From: Characterization of pneumococcal Ser/Thr protein phosphatase phpP mutant and identification of a novel PhpP substrate, putative RNA binding protein Jag

Fig. 4

Cell wall synthesis in ΔphpP mutant. Nascent peptidoglycan synthesis sites in live pneumococcal cells of WT (Sp1), ΔphpP (Sp113) strain and overexpression strain ΔphpP PZn-phpP (Sp120) were stained by fluorescently labelled vancomycin (Van-FL). Overexpression of PhpP in strain ΔphpP PZn-phpP was achieved by addition of 0.3 mM ZnSO4 into C + Y medium. Full arrow shows cell with disturbed nascent peptidoglycan synthesis. Open arrow indicate multiple unconstricted cell division septa. Van-FL signal and its overlay with phase contrast images are shown. Scale bars 1 μm

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BMC Microbiology

ISSN: 1471-2180

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