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Fig. 3 | BMC Microbiology

Fig. 3

From: Characterization of pneumococcal Ser/Thr protein phosphatase phpP mutant and identification of a novel PhpP substrate, putative RNA binding protein Jag

Fig. 3

PhpP is involved in regulation of cell division in S. pneumoniae. a Morphology and cell length analysis of WT strain (Sp1), reverted strain WTR (Sp222) and ΔphpP (Sp113) or ΔstkP (Sp10) strains grown in C + Y medium to mid-exponential phase. b Cell length depends on expression of PhpP in complementation strain ΔphpP PZn-phpP (Sp120). Micrographs of complementation strain cultivated in the presence of 0, 0.2, 0.25 and 0.3 mM ZnSO4 in C + Y medium. Cell length in panel A and B is expressed as a median value ± MAD (n = 300). Bar 5 μm. c Cell length analysis. Cell length parameters measured with MicrobeTracker software were analyzed and plotted in box-and-whiskers graph. Mann-Whitney U test: * cell length of mutant strain is significantly different from that of the WT strain P < 0.0001. 300 cells were scored per sample. d Scanning electron microscopy of WT strain (Sp1), ΔstkP strain (Sp10) and ΔphpP strain (Sp113) cultivated in TSB medium. Magnifications are the same for all panels. Bar 0.5 μm. a-d: Representative data for three independent experiments are shown

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BMC Microbiology

ISSN: 1471-2180

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