Fig. 4From: Analysis and description of the stages of Aspergillus fumigatus biofilm formation using scanning electron microscopyMicrohyphae of the Aspergillus fumigatus clinical isolate biofilm. This structure was observed by scanning electron microscopy (SEM) at an inoculum concentration of 1 × 106 conidia/mL only at 20 h/37 °C of incubation on the in vitro biofilm. Comparing the microhyphae with the size and diameter of the normal hyphae are crucial. a microhyphae projecting between the normal hyphae and the extracellular matrix (ECM) (2,700X); b microhyphae exiting and surrounding the normal hyphae (5,000X); c microhyphae on fungal anastomosis (2,500X). d developing microhyphae from the interior of the normal hyphae (5000X). White pointed arrow: normal hyphae; white arrow: microhyphae; white asterisk: porous ECMBack to article page