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Fig. 4 | BMC Microbiology

Fig. 4

From: Analysis and description of the stages of Aspergillus fumigatus biofilm formation using scanning electron microscopy

Fig. 4

Microhyphae of the Aspergillus fumigatus clinical isolate biofilm. This structure was observed by scanning electron microscopy (SEM) at an inoculum concentration of 1 × 106 conidia/mL only at 20 h/37 °C of incubation on the in vitro biofilm. Comparing the microhyphae with the size and diameter of the normal hyphae are crucial. a microhyphae projecting between the normal hyphae and the extracellular matrix (ECM) (2,700X); b microhyphae exiting and surrounding the normal hyphae (5,000X); c microhyphae on fungal anastomosis (2,500X). d developing microhyphae from the interior of the normal hyphae (5000X). White pointed arrow: normal hyphae; white arrow: microhyphae; white asterisk: porous ECM

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