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Table 1 Primers used in this study

From: DNA-based culture-independent analysis detects the presence of group a streptococcus in throat samples from healthy adults in Japan

Primer

Nucleotide Sequence (5’-3’)

Amplicon size (bp)

Reference

16S rRNA

Fwd-AAGAGAGACTAACGCATGTTAGTAAT

Rev-ATTTTCCACTCCCACCATCA

300

This study

V-Na+-ATPase

Fwd-GTCGATTTTGCCACGTACCG

Rev -TGCATGGTCAACTCAATCATTTGC

121

Hung et al. 2012 [27]

Vir

SBR-AGACATGAGCGCAATGGCAAGTTTATCAAATGGTAATTTTTG

VUF-AAACCGTATCTTTGACGCACTCGAGGACAATTTGCGAGATTAG

Variable

Gardiner et al. 1995 [29]

emm

Primer1 –TATT(C/G)GCTTAGAAAATTAA

Primer 2– GCAAGTTCTTCAGCTTGTTT

Variable

CDC a

emm conserved

Fwd-AATARACASTATTCGCTTAGAAAATTA

Rev-GCTTAGTTTTCTTCTTTGCG

Variable

This study

  1. Abbreviations: Fwd forward, Rev reverse, a CDC Centers for Disease Control and Prevention
  2. For the SBR primer, G (shown in bold) replaces T in the original reference