DNA-based culture-independent analysis detects the presence of group a streptococcus in throat samples from healthy adults in Japan

Table 1 Primers used in this study

Primer	Nucleotide Sequence (5’-3’)	Amplicon size (bp)	Reference
16S rRNA	Fwd-AAGAGAGACTAACGCATGTTAGTAAT Rev-ATTTTCCACTCCCACCATCA	300	This study
V-Na⁺-ATPase	Fwd-GTCGATTTTGCCACGTACCG Rev -TGCATGGTCAACTCAATCATTTGC	121	Hung et al. 2012 [27]
Vir	SBR-AGACATGAGCGCAATGGCAAGTTTATCAAATGGTAATTTTTG VUF-AAACCGTATCTTTGACGCACTCGAGGACAATTTGCGAGATTAG	Variable	Gardiner et al. 1995 [29]
emm	Primer1 –TATT(C/G)GCTTAGAAAATTAA Primer 2– GCAAGTTCTTCAGCTTGTTT	Variable	CDC ^a
emm conserved	Fwd-AATARACASTATTCGCTTAGAAAATTA Rev-GCTTAGTTTTCTTCTTTGCG	Variable	This study

Abbreviations: Fwd forward, Rev reverse, ^a CDC Centers for Disease Control and Prevention
For the SBR primer, G (shown in bold) replaces T in the original reference

ISSN: 1471-2180