Fig. 2

Primer specificity. Representative results of PCRs using GAS-specific 16S rRNA and V-Na⁺-ATPase primers. DNA templates (2 ng) from 11 different GAS strains and 11 other Streptococcus species were used for primer specificity testing. S. pyogenes JRS4 DNA and sterile deionized water were used for the positive and negative controls, respectively. Lane M, DNA marker; P, positive control; N, negative control; 1, emm1; 2, emm3; 3, emm4; 4, emm6; 5, emm11; 6, emm12; 7, emm28; 8, emm58; 9, emm75; 10, emm87; 11, emm89; 12, S. salivarius; 13, S. sanguinis; 14, S. sobrinus; 15, S. suis; 16, S. mutans; 17, S. pneumoniae; 18, S. mitis; 19, S. dysgalactiae; 20, S. constellatus; 21, S. intermedius; 22, S. anginosus