From: Ciprofloxacin triggered glutamate production by Corynebacterium glutamicum
E. coli strains | ||
---|---|---|
DH5α | F− thi-1 endA1 hsdr17(r−, m−) supE44 ∆lacU169 (Φ80lacZ∆M15) recA1 gyrA96 relA1 | [74] |
C. glutamicum strains | ||
 WT | Wild type strain ATCC13032, auxotrophic for biotin | ATCC |
 MB001 | ATCC 13032 with in-frame deletion of prophages CGP1 (cg1507-cg1524), CGP2 (cg1746-cg1752), and CGP3 (cg1890-cg2071) | [75] |
 ARG1 | WT with in-frame deletion of ∆argR carrying the pEKEx-argB fbr vector | [76] |
 ORN1 | WT with in-frame deletion of ∆argFR | [76] |
 DM1729 | WT with lysC P458S, hom V59A, pyc T311I | [70] |
 PUT21 | WT with in-frame deletion of ∆argFR carrying the pVWEx1-speC-argF leaky vector | [77] |
 MB001∆yggB | MB001 with in-frame deletion of ∆yggB | This study |
Plasmids | ||
 pK19mobsacB | Kanr, mobilizable E. coli vector for the construction of insertion and deletion mutants of C. glutamicum (oriV, sacB, lacZ) | [78] |
 pK19∆yggB | KanR, pk19mobsacB with the deletion construct of gene yggB | This study |