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Table 3 Strains and plasmids used in this study

From: Ciprofloxacin triggered glutamate production by Corynebacterium glutamicum

E. coli strains

DH5α

F− thi-1 endA1 hsdr17(r−, m−) supE44 ∆lacU169 (Φ80lacZ∆M15) recA1 gyrA96 relA1

[74]

C. glutamicum strains

 WT

Wild type strain ATCC13032, auxotrophic for biotin

ATCC

 MB001

ATCC 13032 with in-frame deletion of prophages CGP1 (cg1507-cg1524), CGP2 (cg1746-cg1752), and CGP3 (cg1890-cg2071)

[75]

 ARG1

WT with in-frame deletion of ∆argR carrying the pEKEx-argB fbr vector

[76]

 ORN1

WT with in-frame deletion of ∆argFR

[76]

 DM1729

WT with lysC P458S, hom V59A, pyc T311I

[70]

 PUT21

WT with in-frame deletion of ∆argFR carrying the pVWEx1-speC-argF leaky vector

[77]

 MB001∆yggB

MB001 with in-frame deletion of ∆yggB

This study

Plasmids

 pK19mobsacB

Kanr, mobilizable E. coli vector for the construction of insertion and deletion mutants of C. glutamicum (oriV, sacB, lacZ)

[78]

 pK19∆yggB

KanR, pk19mobsacB with the deletion construct of gene yggB

This study