Cobalamin production by Lactobacillus coryniformis: biochemical identification of the synthetized corrinoid and genomic analysis of the biosynthetic cluster

Table 1 Comparative analysis of the deduced amino acid sequences of each ORF involved in vitamin B₁₂ biosynthesis cluster of Lactobacillus coryniformis CRL 1001 with those present in other cobalamin producer strains

L. coryniformis CRL 1001		Lactobacillus rossiae		Lactobacillus reuteri		Listeria sp.		Salmonella sp.
Protein	Length (aa)	Length (aa)	Identity (%)	Length (aa)	Identity (%)	Length (aa)	Identity (%)	Length (aa)	Identity (%)
CobA	188	189	71	194	68	188	56	176	40
Cb1 T	166	174	61	-*	-*	164	54	192	36
Cob D	360	364	41	362	42	361	45	364	42
Pdu X	288	-*	-*	-*	-*	291	37	288	36
Cbi A	456	457	59	454	61	452	54	459	50
Cbi B	312	319	62	319	58	316	55	459	50
Cbi C	217	213	68	228	64	210	64	210	51
Cbi D	365	375	74	383	73	373	55	370	50
Cbi E	199	200	54	199	55	198	53	201	43
Cbi T	184	188	56	184	63	189	53	192	44
Cbi F	254	258	83	253	77	249	76	257	71
Cbi G	351	355	55	351	53	343	46	351	36
Cbi H	241	241	77	241	78	241	67	241	58
Cbi J	249	250	47	252	56	250	45	263	33
Hem D/Cob A	449	468	45	464	48	493	40	-*	-*
Cbi K	262	259	47	259	53	261	49	264	46
Cbi L	235	232	47	232	62	236	48	237	38
Cbi M	248	239	77	247	74	244	59	245	57
Cbi N	104	114	76	103	63	82	56	93	45
Cbi Q	235	230	54	225	56	225	37	225	34
Cbi O	276	274	65	269	63	268	54	271	51
Cbi P	499	499	67	503	70	511	57	506	56
Cys G	153	148	45	152	51	146	44	154	32
Hem A	429	431	53	421	55	-	-	-	-
Hem C	305	309	55	305	55	-	-	-	-
Hem B	324	321	81	323	77	-	-	-	-
Hem L	430	430	77	431	75	-	-	-	-
Cbl S	242	252	53	-*	-*	252	38	273	37
Cob U	196	197	46	196	54	185	41	181	40
Cob S	253	257	52	253	53	248	38	244	35
Cob C	194	194	46	196	43	191	32	202	29
Hem D	209	243	46	236	49	-	-	-	-

Length variation among deduced aminoacid sequences of each ORF related to cobalamin production in Lactobacillus, Salmonella and Listeria strains. The percentage of protein sequence identity is related to those detected in Lactobacillus coryniformis CRL1001. (-*) ORF not detected. (-) ORF not detected among cbi-cob-hem cluster

ISSN: 1471-2180