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Fig. 2 | BMC Microbiology

Fig. 2

From: In vivo functional and molecular characterization of the Penicillin-Binding Protein 4 (DacB) of Pseudomonas aeruginosa

Fig. 2

D,D-peptidase activities on peptidoglycan of Escherichia coli. HPLC chromatograms of peptidoglycan obtained from strains E. coli DV900(DE3)/pET-PBP4HNC, E. coli DV900(DE3)/pET-PBP4HN and E. coli DV900(DE3)/pET-PBP4HC, uninduced (NI) and induced by IPTG (IPTG). Chromatograms for the lysogenized mutant strain E. coli DV900(DE3) and transformed with the expression vector pET-28b(+) are presented as controls. The following muropeptides are identified: M3, monomer disaccharide tripeptide; M4, monomer disaccharide tetrapeptide; M5, monomer disaccharide pentapeptide; M4N, anhydrous monomer disaccharide tetrapeptide; D44, dimer disaccharide tetrapeptide-tetrapeptide; D45, dimer disaccharide tetrapeptide-pentapeptide; D45N, anhydrous dimer disaccharide tetrapeptide-pentapeptide; T445, trimer tetrapeptide-tetrapeptide-pentapeptide; T445N, anhydrous trimer tetrapeptide-tetrapeptide-pentapeptide. A204, absorbance at 204 nm, arbitrary units

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BMC Microbiology

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