Fig. 7From: Crystal structure and DNA binding activity of a PadR family transcription regulator from hypervirulent Clostridium difficile R20291EMSA of cdPadR1WT and cdPadR1W94A binding P cdpadR1 . Protein-free controls are indicated with a minus sign (-). 100 bp P cdpadR1 DNA (0.05 μM) was used in EMSA to determine binding of cdPadR1 to its own promoter at increasing concentrations (0.25, 0.50, 1.0, and 2.0 μM) of proteinBack to article page