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Fig. 6 | BMC Microbiology

Fig. 6

From: Crystal structure and DNA binding activity of a PadR family transcription regulator from hypervirulent Clostridium difficile R20291

Fig. 6

EMSAs of cdPadR1 binding the cdpadR1 promoter (P cdpadR1 ), a norV promoter (P norV ), and b obg promoter (P obg ). The predicted -10 and -35 sites are indicated in blue boxes above the sequence. Orange arrows indicate the inverted repeats TACT/AGTA. c-e Final P cdpadR1, P norV , and P obg (100 bp) concentration in the reaction was 0.1 μM. cdPadR1 was 5, 10, 20, and 40-fold excess over dsDNA. The minus (-) lane contains dsDNA and no cdPadR1. Shifted DNA-protein complexes are annotated with a black arrow and unbound dsDNA migration is marked with a red arrow. f For this EMSA, the final heparin concentration was 4-fold of the standard EMSA concentration used throughout this research. The + lane contains 40-fold excess cdPadR1 over dsDNA

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BMC Microbiology

ISSN: 1471-2180

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