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Table 2 Primers used for mutant and complement strain construction

From: Functional analysis of PrkA - a putative serine protein kinase from Mesorhizobium alhagi CCNWXJ12-2 - in stress resistance

Primer

Sequencea (5′-3′)

PrkA-US

CGGAATTCTCCTTCGTTCGCGGA

PrkA-UA

CGGGATCCGACCTCGTTGCCGA

PrkA-DS

CGGGATCCGCCTTCCCATATCAG

PrkA-DA

GCTCTAGACACTTCCTCCGTGTCGT

CPA

ATGAAGTCGCTTAATCCCCCGGGCATCGGGTCCGGCGCCGGATCGGG

CPB

TAACAAAATATTAACGCCCCGGGTCAGCCCGCCTTGTTGACGCGCATG

  1. aRestriction enzyme sites are underlined
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BMC Microbiology

ISSN: 1471-2180

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