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Table 1 Bacterial strains and plasmids used in this study

From: Functional analysis of PrkA - a putative serine protein kinase from Mesorhizobium alhagi CCNWXJ12-2 - in stress resistance

Strain or plasmid

Descriptiona

Source or reference

Escherichia coli

 DH5α

endA hsdR17 supE44 thi-1 recA1 gyrA relA1 Δ(lacZYA-argF)U169 deoR [Φ80 dlacΔ(lacZ)M15]

[27]

 S17-1λpir

Tpr Strr recA thi pro hsdR hsdM + RP4::2-Tc::Mu::Km Tn7 λpir lysogen

[28]

 DH-PrkA

DH5α carrying pK18prkA

This study

 DH-CPrkA

DH5α carrying pBLprkA

This study

Mesorhizobium alhagi

 XJ12-2

Wild-type

[18]

ΔprkA

XJ12-2 ΔprkA

This study

 CΔprkA

ΔprkA carrying pBLprkA

This study

Plasmids

 pK18mobsacB

Suicide vector derived from plasmid pK18; Mob+ sacB Kmr

[25]

 pBBR1MCS-5

Broad-host-range cloning vector; Gmr

[29]

 pK18prkA

pK18mobsacB::prkA

This study

 pBLprkA

pBL carrying prkA

This study

  1. aKmr, kanamycin resistance; Gmr, gentamicin resistance