Strain or plasmid | Descriptiona | Source or reference |
---|---|---|
Escherichia coli | ||
DH5α | endA hsdR17 supE44 thi-1 recA1 gyrA relA1 Δ(lacZYA-argF)U169 deoR [Φ80 dlacΔ(lacZ)M15] | [27] |
S17-1λpir | Tpr Strr recA thi pro hsdR hsdM + RP4::2-Tc::Mu::Km Tn7 λpir lysogen | [28] |
DH-PrkA | DH5α carrying pK18prkA | This study |
DH-CPrkA | DH5α carrying pBLprkA | This study |
Mesorhizobium alhagi | ||
XJ12-2 | Wild-type | [18] |
ΔprkA | XJ12-2 ΔprkA | This study |
CΔprkA | ΔprkA carrying pBLprkA | This study |
Plasmids | ||
pK18mobsacB | Suicide vector derived from plasmid pK18; Mob+ sacB Kmr | [25] |
pBBR1MCS-5 | Broad-host-range cloning vector; Gmr | [29] |
pK18prkA | pK18mobsacB::prkA | This study |
pBLprkA | pBL carrying prkA | This study |