Skip to main content

Table 1 Bacterial strains and plasmids used in this study

From: Functional analysis of PrkA - a putative serine protein kinase from Mesorhizobium alhagi CCNWXJ12-2 - in stress resistance

Strain or plasmid Descriptiona Source or reference
Escherichia coli
 DH5α endA hsdR17 supE44 thi-1 recA1 gyrA relA1 Δ(lacZYA-argF)U169 deoR [Φ80 dlacΔ(lacZ)M15] [27]
 S17-1λpir Tpr Strr recA thi pro hsdR hsdM + RP4::2-Tc::Mu::Km Tn7 λpir lysogen [28]
 DH-PrkA DH5α carrying pK18prkA This study
 DH-CPrkA DH5α carrying pBLprkA This study
Mesorhizobium alhagi
 XJ12-2 Wild-type [18]
ΔprkA XJ12-2 ΔprkA This study
 CΔprkA ΔprkA carrying pBLprkA This study
Plasmids
 pK18mobsacB Suicide vector derived from plasmid pK18; Mob+ sacB Kmr [25]
 pBBR1MCS-5 Broad-host-range cloning vector; Gmr [29]
 pK18prkA pK18mobsacB::prkA This study
 pBLprkA pBL carrying prkA This study
  1. aKmr, kanamycin resistance; Gmr, gentamicin resistance