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Fig. 5 | BMC Microbiology

Fig. 5

From: Differential protection by cell wall components of Lactobacillus amylovorus DSM 16698Tagainst alterations of membrane barrier and NF-kB activation induced by enterotoxigenic F4+ Escherichia coli on intestinal cells

Fig. 5

Inhibition of F4+ETEC-induced NF-kB activation by S-layer protein–coated or uncoated -cell wall fragments. Caco-2/TC7 cells were untreated (control, C), infected with ETEC (E), treated with S-CWF or U-CWF, co-treated with S-CWF or U-CWF and E (S-CWF + E or U-CWF + E, respectively), pre-treated with S-CWF or U-CWF before F4+ETEC infection (prS-CWF + E or prU-CWF + E, respectively). Cell lysates were fractionated by SDS-PAGE and transferred to nitrocellulose filters. Membranes were incubated with rabbit polyclonal anti-p65 or anti-phospho (P)-p65 primary antibodies and then with horseradish peroxidase-conjugated secondary antibodies. The figure shows the densitometric values of phospho (P)-p65 protein, normalized to its corresponding unphosphorylated form. Values represent means ± SD of three independent experiments, carried out in triplicate. Statistically significant differences are shown and * indicates P < 0.001 from the control group, # indicates P < 0.05 from the ETEC group

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