Fig. 4From: Production of infectious HCV genotype 1b virus in cell culture using a novel Set of adaptive mutationsNeutralization of the cell culture by treatment with a CD81-specific antibody. ALS32.50 cells were inoculated with culture medium containing approximately 1300 FFU/mL in the presence of a CD81-specific antibody (anti-CD81, gray bar), polymerase inhibitor (2′CMeA, black bar), or a control antibody (CTRL, white bar), and were incubated for 96 h. Inoculated cells were analyzed by qRT-PCR and sandwich EIA. Values obtained with FFU in the presence of CTRL were set to 100 %. All assays were conducted two independent experiments, and data are represented by an averageBack to article page