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Table 5 Study of LcpA topology: LcpA-BlaM hybrid characterization for antibiotic single cell resistance

From: Enterococcus hirae LcpA (Psr), a new peptidoglycan-binding protein localized at the division site

  Single cell test
Strains E. coli Top10/pDML1677 E. coli Top10 F’/pCIPblaM
Characteristics lcpA-blaM hybrid Δ blaM
Kma Resistant Resistant
Kma + Ampb + IPTGc Resistant Sensitive
MIC-Value (μg.ml−1) >100 2
β-lactamase moiety location Outside the cell -
  1. a Kanamycin : 50 μg.ml−1, b Ampicillin: 10 μg.ml−1, c IPTG: 100 μM