Table 5 Study of LcpA topology: LcpA-BlaM hybrid characterization for antibiotic single cell resistance
From: Enterococcus hirae LcpA (Psr), a new peptidoglycan-binding protein localized at the division site
Single cell test | ||
|---|---|---|
Strains | E. coli Top10/pDML1677 | E. coli Top10 F’/pCIPblaM |
Characteristics | lcpA-blaM hybrid | Δ blaM |
Kma | Resistant | Resistant |
Kma + Ampb + IPTGc | Resistant | Sensitive |
MIC-Value (μg.ml−1) | >100 | 2 |
β-lactamase moiety location | Outside the cell | - |