Table 1 Bacterial strains and plasmids used in this study
From: Enterococcus hirae LcpA (Psr), a new peptidoglycan-binding protein localized at the division site
Strain or plasmid | Genotype and/or relevant characteristic | Source or reference |
|---|---|---|
Strains | ||
E.coli DH5α | F– Φ80lacZΔM15 Δ (lacZYA-argF) U169 recA1 endA1 hsdR17 (rK–, mK+) phoA supE44 λ– thi-1 gyrA96 relA1 | Invitrogen |
E. coli Top10 | F– mcrA Δ (mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ (ara leu) 7697 galU galK rpsL (StrR) endA1 nupG | Invitrogen |
E. coli Rosetta (DE3) pLysS | F− ompT hsdS B (rB − mB −) gal dcm (DE3) pLysSRARE (CamR) | Novagen |
E. hirae ATCC 9790 | Wild-type strain (MIC of PenG : 0.6 μg/ml) | American Type Culture Collection |
E. hirae FS4 | ATCC 9790 psr::Kmr (substitution) | Sapunaric et al., 2003 [22] (17) |
E. hirae R40 | Penr mutant derived from ATCC 9790 (MIC of PenG : 60 μg/ml) | Fontana et al., 1983 [7] (5) |
Plasmids | ||
pET-22b | Expression vector, ampr | Novagen |
pET-22b-lcpA | Expression vector carrying lcpA, ampr | This study |
pET-22b-lcpA 30–293 | Expression vector carrying the truncated form of lcpA gene, ampr | This study |
pCIPblaM | Reporter vector using TEM β-lactamase, Kmr | Chahbounel et al., 2005 [40] (27) |
pDML1677 | Reporter vector carrying lcpA-blaM in-frame fusion, Kmr | This study |