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Table 1 Bacterial strains and plasmids used in this study

From: Enterococcus hirae LcpA (Psr), a new peptidoglycan-binding protein localized at the division site

Strain or plasmid Genotype and/or relevant characteristic Source or reference
Strains
E.coli DH5α F– Φ80lacZΔM15 Δ (lacZYA-argF) U169 recA1 endA1 hsdR17 (rK–, mK+) phoA supE44 λ– thi-1 gyrA96 relA1 Invitrogen
E. coli Top10 F– mcrA Δ (mrr-hsdRMS-mcrBC) Φ80lacZΔM15 ΔlacX74 recA1 araD139 Δ (ara leu) 7697 galU galK rpsL (StrR) endA1 nupG Invitrogen
E. coli Rosetta (DE3) pLysS F ompT hsdS B (rB mB ) gal dcm (DE3) pLysSRARE (CamR) Novagen
E. hirae ATCC 9790 Wild-type strain (MIC of PenG : 0.6 μg/ml) American Type Culture Collection
E. hirae FS4 ATCC 9790 psr::Kmr (substitution) Sapunaric et al., 2003 [22] (17)
E. hirae R40 Penr mutant derived from ATCC 9790 (MIC of PenG : 60 μg/ml) Fontana et al., 1983 [7] (5)
Plasmids
 pET-22b Expression vector, ampr Novagen
 pET-22b-lcpA Expression vector carrying lcpA, ampr This study
 pET-22b-lcpA 30–293 Expression vector carrying the truncated form of lcpA gene, ampr This study
 pCIPblaM Reporter vector using TEM β-lactamase, Kmr Chahbounel et al., 2005 [40] (27)
 pDML1677 Reporter vector carrying lcpA-blaM in-frame fusion, Kmr This study