Fig. 2
From: Enterococcus hirae LcpA (Psr), a new peptidoglycan-binding protein localized at the division site
a SDS-PAGE analysis of LcpA overproduction in E. coli Rosetta DE3 pLysS cells. Total fractions of induced (I) and non induced (NI) cultures transformed with pET-22b or pET-22b-lcpA underwent electrophoresis. The arrow shows the overproduction of LcpA. MW – Molecular Weight Marker. b SDS-PAGE analysis of the soluble (S) and membrane (M) fractions. Purification of recombinant LcpA by Ni2+-NTA chromatography (Ni2+-NTA). The arrows show the LcpA protein. MW – Molecular Weight Marker. c Purification of recombinant LcpA30–293. SDS-PAGE analysis after first step of purification by Ni2+-NTA chromatography (Ni2+-NTA) and after second step of purification by ion-exchange chromatography (Ni2+-NTA + Ion exch.). MW – Molecular Weight Marker