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Fig. 6 | BMC Microbiology

Fig. 6

From: Mycoplasma ovipneumoniae induces sheep airway epithelial cell apoptosis through an ERK signalling-mediated mitochondria pathway

Fig. 6

Induction of the expression of Cyt-c in sheep airway epithelial cells infected with M. ovipneumoniae. Sheep airway epithelial cells cultured on an air-liquid interface model were apically infected with M. ovipneumoniae (MO) at an MOI of 100 for 24 h, changes in the expression of mitochondrial Cyt-c were assessed by an immunoblotting assay. a Representative images of immunoblots of Cyt-c and β-actin, revealed an increased release of Cyt-c in cells infected with MO, and the increased level of Cyt-c was reduced in the presence of MEK/ERK signalling PD980025 or ROS scavenger NAC. b Semi-quantitative analysis of the expression of proteins in (a) by evaluating relative densitometric densities using arbitrary units (A.U.), which was calculated with densitometric signal of a protein of interest over that of the corresponding β-actin internal control

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