Fig. 5

Induction of the expression of BCL-2 family in sheep airway epithelial cells infected with M. ovipneumoniae. Sheep airway epithelial cells cultured on an air-liquid interface model were apically infected with M. ovipneumoniae (MO) at an MOI of 100 for 24 h, changes in the expression of BCL-2 family members was assessed using an immunoblotting assay. a Representative images of immunoblots of indicated proteins, revealed an evoked expression of pro-apoptotic proteins Bad, Bcl-2, Bcl-xl and Bak, and the MO-induced expression of these proteins could be diminished by MEK/ERK signalling PD980025 or ROS scavenger NAC. b Semi-quantitative analysis of the expression of proteins in (a) by evaluating relative densitometric densities using arbitrary units (A.U.), calculated based on the densitometric signal of a protein of interest over that of the corresponding β-actin internal control