Fig. 3
Effects of M. ovipneumoniae infections on CAT activity and GSS expression. Sheep airway epithelial cells cultured on an air-liquid interface model were apically infected with 1, 10 and 100 MOI of M. ovipneumoniae (MO) at 37 °C for 24 h, and the activities of CAT and GSS expression were determined. a A dose-dependent decrease of CAT activity was observed in airway epithelial cells infected with MO. b A representative image of immunoblot showed the reduced expression of GSS protein in ALI cells infected with MO (MOI = 100) (top panel) compared with the uninfected cells, which was significantly different based on the value of densitometric arbitrary units (A.U.), which was calculated based on densitometric signal of GSS protein over that of the corresponding β-actin internal control (bottom panel). Data were expressed as the means ± SD from three independent experiments, with each experimental had six replicated ALI cultures (N = 18) in A. Compared with the uninfected controls, *: p < 0.05; **: p < 0.01. The cell numbers of each transwell with diameter of 24 mm was determined as 107