Skip to main content
Fig. 1 | BMC Microbiology

Fig. 1

From: Mycoplasma ovipneumoniae induces sheep airway epithelial cell apoptosis through an ERK signalling-mediated mitochondria pathway

Fig. 1

Impact of M. ovipneumoniae infection on the cell death and mitochondrial membrane potential of sheep airway epithelial cells. Sheep airway epithelial cells cultured on an air-liquid interface model were apically infected with 1, 10 and 100 MOI of M. ovipneumoniae (MO) at 37 °C for 24 h. The cell viability was detected in terms of a LDH assay (a) and the mitochondrial membrane potential was determined using a potential-sensing fluorescent probe (JC-1) (b). a The percentage of cell death of ALI sheep airway epithelial cells infected with the indicated doses of MO, and a dose-dependent cell death was induced by MO infections. b Mitochondrial membrane potential (ΔΨm) of airway epithelial cells infected with indicated dose of MO, and the fluorescence intensity of both mitochondrial JC-1 monomers (λex 514 nm, λem 529 nm) and aggregates (λex 585 nm, λem 590 nm) were measured. The mitochondrial ΔΨm of airway epithelial cells were calculated as the fluorescence ratio of red over green. The mitochondrial ΔΨm decreased with increasing MOI of infection. Data were expressed as the means ± SD of three independent experiments, and each experiment had six replicated ALI cultures (N = 18). Compared with the uninfected controls, *: p < 0.05; **: p < 0.01. The cell numbers of each transwell with diameter of 24 mm was determined as 107

Back to article page