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Fig. 3 | BMC Microbiology

Fig. 3

From: Erratum to: Genotyping bacterial and fungal pathogens using sequence variation in the gene for the CCA-adding enzyme

Fig. 3

Multiplex PCR with individual fluorescence-labeled primers for different Vibrio strains. a. Species-specific amplification of the flexible loop-encoding DNA sequence. Indicated amounts of individual genomic DNA (1: V. alginolyticus, 2: V. parahaemolyticus, 3: V. vulnificus) were added to the primer mix. PCR products were visualized in the agarose gel by the different fluorescence of the species-specific primers. Down to 10 pg of each DNA sample were readily detected, without any cross reactivity with the other genomes. N, negative control. b. Human DNA does not interfere with the specific detection of Vibrio DNA. 0.1 ng of genomic DNA of the indicated Vibrio strains were mixed with a 500 to 1000-fold excess (50 and 100 ng) of human genomic DNA in a multiplex PCR and visualized as above. Compared to the positive control (0, no human DNA added), no additional bands appeared, indicating an exclusive and highly specific amplification of Vibrio DNA only. N, negative control with 50 ng of human genomic DNA

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