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Table 4 Twenty-trial Plackett–Burman experimental design for evaluation of independent variables with coded values along with the observed cholesterol oxidase activity

From: Identification and statistical optimization of fermentation conditions for a newly isolated extracellular cholesterol oxidase-producing Streptomyces cavourensis strain NEAE-42

Std

Run no.

Coded levels of independent variables

Cholesterol oxidase activity (U/mL)

Residuals

A

B

C

D

E

F

G

H

J

K

L

M

N

O

P

Dummy 1

Dummy 2

Dummy 3

Dummy 4

Actual value

Predicted value

15

1

1

1

1

−1

1

−1

1

−1

−1

−1

−1

1

1

−1

1

1

−1

−1

1

0.397

0.297

0.100

6

2

−1

−1

1

1

−1

1

1

−1

−1

1

1

1

1

−1

1

−1

1

−1

−1

1.522

1.622

−0.100

4

3

1

1

−1

1

1

−1

−1

1

1

1

1

−1

1

−1

1

−1

−1

−1

−1

6.910

6.907

0.003

3

4

1

−1

1

1

−1

−1

1

1

1

1

−1

1

−1

1

−1

−1

−1

−1

1

1.267

1.263

0.003

7

5

−1

−1

−1

1

1

−1

1

1

−1

−1

1

1

1

1

−1

1

−1

1

−1

6.000

5.900

0.100

17

6

−1

1

1

1

1

−1

1

−1

1

−1

−1

−1

−1

1

1

−1

1

1

−1

1.080

1.180

−0.100

18

7

−1

−1

1

1

1

1

−1

1

−1

1

−1

−1

−1

−1

1

1

−1

1

1

2.325

2.225

0.100

8

8

−1

−1

−1

−1

1

1

−1

1

1

−1

−1

1

1

1

1

−1

1

−1

1

1.953

2.053

−0.100

14

9

1

1

−1

1

−1

1

−1

−1

−1

−1

1

1

−1

1

1

−1

−1

1

1

2.011

2.008

0.003

19

10

1

−1

−1

1

1

1

1

−1

1

−1

1

−1

−1

−1

−1

1

1

−1

1

2.260

2.263

−0.003

20

11

−1

−1

−1

−1

−1

−1

−1

−1

−1

−1

−1

−1

−1

−1

−1

−1

−1

−1

−1

0.690

0.687

0.003

2

12

−1

1

1

−1

−1

1

1

1

1

−1

1

−1

1

−1

−1

−1

−1

1

1

0.490

0.487

0.003

9

13

1

−1

−1

−1

−1

1

1

−1

1

1

−1

−1

1

1

1

1

−1

1

−1

0.200

0.100

0.100

16

14

1

1

1

1

−1

1

−1

1

−1

−1

−1

−1

1

1

−1

1

1

−1

−1

0.000

0.003

−0.003

10

15

−1

1

−1

−1

−1

−1

1

1

−1

1

1

−1

−1

1

1

1

1

−1

1

6.600

6.603

−0.003

12

16

−1

1

−1

1

−1

−1

−1

−1

1

1

−1

1

1

−1

−1

1

1

1

1

1.667

1.670

−0.003

1

17

1

1

−1

−1

1

1

1

1

−1

1

−1

1

−1

−1

−1

−1

1

1

−1

4.787

4.887

−0.100

13

18

1

−1

1

−1

1

−1

−1

−1

−1

1

1

−1

1

1

−1

−1

1

1

1

2.076

2.176

−0.100

5

19

−1

1

1

−1

1

1

−1

−1

1

1

1

1

−1

1

−1

1

−1

−1

−1

3.497

3.397

0.100

11

20

1

−1

1

−1

−1

−1

−1

1

1

−1

1

1

−1

−1

1

1

1

1

−1

0.807

0.810

−0.003

  1. The “-1” sign correspond to the minimum value and the “ + 1” sign correspond to the maximum value of the input parameter range