Identification and statistical optimization of fermentation conditions for a newly isolated extracellular cholesterol oxidase-producing Streptomyces cavourensis strain NEAE-42

Table 10 Fit summary for experimental data

Sequential Model Sum of Squares
Source	Sum of Squares	df	Mean Square	F-value	P-value (Prob > F)
Linear vs Mean	47.829	3	15.943	0.785	0.5194
Two factors interaction (2FI) vs Linear	10.748	3	3.583	0.148	0.9289
Quadratic vs 2FI	306.352	3	102.117	132.900	<0.0001*
Residual	2.226	6	0.371
Lack of Fit Tests
Source	Sum of Squares	df	Mean Square	F-value	P-value (Prob > F)
Linear	324.750	11	29.523	4498.259	<0.0001*
Two factors interaction (2FI)	314.003	8	39.250	5980.409	<0.0001*
Quadratic	7.651	5	1.530	233.149	<0.0001*
Pure Error	0.033	5	0.007
Model Summary Statistics
Source	Standard deviation	R-Squared	Adjusted R-Squared	Predicted R-Squared	PRESS
Linear	4.505	0.128	−0.035	−0.277	475.939
Two factors interaction (2FI)	4.915	0.157	−0.232	−0.826	680.540
Quadratic	0.877	0.979	0.961	0.843	58.334

ISSN: 1471-2180