Fig. 4

Contribution of FHA and PRN in B. pertussis adhesion to A549 cells. a A549 cells were infected with labeled B. pertussis strains BP536 (wild-type), BP102 (Δfha) and BBC42 (Δprn) at OD₆₀₀ 0.1 for 1 h. After washes, cell-associated bacteria were quantified by fluorescence reading at Ex/Em 485/535 nm. Experiments were done in triplicates and data represent mean ± SD. b Adhesion assay was performed as described in A. Infected cells were fixed and actin (phalloidin, red) and nuclei (DAPI, blue) were stained for confocal microscopy analysis. Images are 3D view of Z-stack acquisition. Results represent mean ± SD from a representative experiment performed in triplicates